I pulled them all at once. I'm using 4" PVC external (buried) and 3/4 flex internally.
What I did was I pulled a 1/4" nylon rope through from end to end for measurement, placing a piece of electrical tape at the final length. Then removed the rope from the run and add 4ft (2ft per side) to be safe. Then I used this rope and marker to unspool 5 lengths (2 hot, 2 neutral and 1 ground). Taped them together with mule tape and the ends staggered 6" to allow better flex around corners.
One person pulls the mule tape LIGHTLY while the other pushes the lines into the conduit. You should use lube. Don't skip this step. It makes everything easier and ensures you don't melt the PVC through friction. Do not rush the process the faster you push it the more likely you are to damage something or get hung up . The harder you pull it the more likely you are to friction melt the wire or conduit.
To get the rope through the conduit use kite string with a plastic grocery bag tied to it. Shove in one side of conduit. Got to other side and vacuum the string through. Use the string to pull the rope/make tape